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Key Documents

MABF978

Sigma-Aldrich

Anti-GD3, 9-O-acetyl Antibody, clone UM4D4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

antibody form

purified antibody

Quality Level

clone

UM4D4
monoclonal

species reactivity

mouse, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

activity assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable

isotype

IgMκ

General description

Human rheumatoid synovial T cell line ST-1.

Specificity

Clone UM4D4 detects 9-O-acetylated ganglioside GD3. GD3 is also known as CD60a. 9-O-acetylated GD3 is known as CDw60 or CD60b, while 7-O-acetylated GD3 is known as CD60c.

Application

Epitope: 9-O-acetylated disialosyl group on ganglioside GD3.

Quality

Evaluated by Flow Cytometry in human PBMCs.

Flow Cytometry Analysis: 1 µg of this antibody detected CDw60-positive human PBMCs.

Physical form

Purified mouse monoclonal IgMκ antibody in PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Human. Braod species reactivity expected. Target structure is not species-specific.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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