MABF3160

Anti-N-Cadherin Antibody, clone 8C11

• Synonym(s): Cadherin-2;CDw325;Neural Cadherin;CD325
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 8C11, monoclonal
• mol wt: calculated mol wt 99.81 and 97.04 kDa; observed mol wt ~105 and 130 kDa
• purified by: using protein G
• species reactivity: hamster, human
• packaging: antibody small pack of 100 μg
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; inhibition assay: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_001295105
• UniProt accession no.: P19022
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... CHD2(1000)

Description

General description

Cadherin-2 (UniProt: P19022; also known as CDw325, Neural cadherin, N-cadherin, CD325) is encoded by the CDH2 (also known as CDNH, NCAD) gene (Gene ID: 1000) in human. N-Cadherin is a single-pass type I membrane glycoprotein protein that plays a role in calcium-dependent cell adhesion. Its expression is observed in cells of the nervous tissue, vascular smooth muscle cells, and myocytes. N-cadherins preferentially interacts with themselves in a homophilic manner in connecting cells; may thus contribute to the sorting of heterogeneous cell types. N-cadherin are synthesized as precursor proteins with a signal peptide (aa 1-25) and a propeptide (aa 26-159) that are proteolytically cleaved to generate functional, mature protein. Newly synthesized proN-cadherin (aa 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. The mature N-Cadherin contains an extracellular domain (aa 160-724) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (aa 725-745), and a conserved cytoplasmic domain (aa 746-906) that interacts with catenins to link cadherins to the actin cytoskeleton. N-cadherin is well expressed in endothelial cells and plays an essential role in the maturation and stabilization of normal vessels. However, it also promotes tumor cell survival, migration and invasion, and a high level of its expression is often associated with poor prognosis. A 69 amino acid segment of EC-4 of N-cadherin is reported to be essential and sufficient to increase cell motility and promote epithelial to mesenchymal transition. (Ref.: Kim, JB., et al. (2000). J. Cell Biol. 151(6); 1193-1205; Navarro, P., et al. (1998). J. Cell Biol. 140(6); 1475-1484).

Specificity

Clone 8C11 is a mouse monoclonal antibody that detects N-Cadherin. It targets an epitope within the extracellular domain.

Immunogen

MBP-tagged recombinant fragment corresponding to 502 amino acids from the extracellular domain of human N-Cadherin.

Application

Quality Control Testing

Evaluated by Western Blotting in Human heart tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Cadherin-2 in Human heart tissue lysate.

Tested applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected N-Cadherin in Human brain and cerebellum tissue extracts.

Western Blotting Analysis: A representative lot detected N-Cadherin in Western Blotting applications (Kim, J.B., et al. (2000). J Cell Biol. 151(6):1193-1206).

Flow Cytometry Analysis: 1 μg from a representative lot detected N-Cadherin in one million KG-1 cells.

Inhibition Assay: A representative lot inhibited motility of BT20 breast cancer cells (Kim, J.B., et al. (2000). J Cell Biol. 151(6):1193-1206).

Flow Cytometry Analysis: A representative lot detected N-Cadherin in Flow Cytometry applications (Wahlig, S., et al. (2020). Sci Rep. 10(1):1681).

Immunocytochemistry Analysis: A representative lot detected Cadherin-2 in Immunocytochemistry applications (Navarro, P., et al. (1998). J Cell Biol.;140(6):1475-1484).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-N-Cadherin, clone 8C11, Cat. No. MABF3160, is a mouse monoclonal antibody that detects N-Cadherin and is tested for use in Flow Cytometry, Immunocytochemistry, Inhibition Assay, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable