HNCSMAG-35K

MILLIPLEX^® Human Circadian/Stress Magnetic Bead Panel - Multiplex Assay

• UNSPSC Code: 12161503
• eCl@ss: 32161000

Properties

• description: The MILLIPLEX^® MAP Human Circadian/Stress Panel is a 2-plex kit containing all reagents needed for simultaneous quantification of Cortisol and/or Melatonin in serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples on Luminex systems.
• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 129%; (Spike Recovery for Melatonin); sensitivity: 4.3 pg/mL; (Melatonin; MinDC+2SD); sensitivity: 686 pg/mL; (Cortisol; MinDC+2SD); standard curve range: 3.4-2,500 pg/mL; (Melatonin); standard curve range: 686-500,000 pg/mL; (Cortisol); inter-assay cv: <25%; intra-assay cv: <15%
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

The MILLIPLEX^® Human Circadian/Stress Bead Panel enables you to focus on the relevance and therapeutic potential of Melatonin and Cortisol in your research model. Coupled with the Luminex^® xMAP^® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.

The MILLIPLEX^® Human Circadian/Stress Bead Panel is to be used for the simultaneous quantification of Cortisol and Melatonin. This is a configurable assay, and either or both analytes can be selected. This kit may be used for the analysis of human serum, plasma, tissue/cell lysate, culture supernatant samples, or cerebrospinal fluid (CSF). Serum or plasma samples should be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with this kit.

Panel Type: Neuroscience

Specificity

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Application

• Analytes: Cortisol, Melatonin
• Recommended Sample type: serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples
• NOTE: Sample extraction protocols are required for serum/plasma samples. Other sample types (e.g. CSF, culture supernatant, saliva, etc.) do not require sample extraction.
• Recommended Sample dilution: 1:4 for extracted serum and plasma or neat for CSF and culture supernatant
• Assay Run Time: Overnight
• Research Category: Neuroscience Metabolism
• Research Subcategory: Category Metabolic Disorders Obesity Inflammation & Autoimmune Mechanisms
• NOTE: This is a competitive assay

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96-well plate

Everything you need in a single kit.

Components

Human Circadian/Stress Standard, 1 vial, lyophilized

Human Circadian/Stress Quality Control 1, 1 vial, lyophilized

Human Circadian/Stress Quality Control 2, 1 vial, lyophilized

Assay Buffer, 2 bottles, 30 mL ea

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Human Circadian/Stress Primary Antibodies, 1 bottle, 3.5 mL

Human Circadian/Stress Detection Antibodies, 1 bottle, 5.5 mL

Human Circadian/Stress Antibody-Immobilized Magnetic Beads, 20X, 200 uL ea

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate with 2 Foil Plate Sealers

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.