AB2251-I
Anti-VGluT2 Antibody
serum, from guinea pig
Synonym(s):
Vesicular glutamate transporter 2, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporte, Solute carrier family 17 member 6, VGluT2
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
guinea pig
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
rat ... Slc17A6(84487)
General description
Vesicular glutamate transporter 2 (UniProt Q9JI12; also known as Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporte, Solute carrier family 17 member 6, VGluT2) is encoded by the Slc17a6 (also known as Dnpi, Vglut2) gene (Gene ID 84487) in rat species. Vesicular glutamate transporters (VGluTs) are proton-dependent carriers responsible for the upload of the major excitatory neurotransmitter glutamate into synaptic vesicles within presynaptic terminals to allow later regulated release at the synaptic cleft. Studies conducted in mice show that VGluT1 and VGluT2 are essential for vital functions, while Vglut3-knockout mice have a normal life expectancy despite being deaf and developing anxiety and discrete basal locomotor phenotypes. VgluT1- and VGluT2-expressing neurons represent the bulk of canonical glutamatergic neurons in the brain, with VgluT1 representing the major subtype and accounting for approximately 80% of total vesicular transport of glutamate in the brain. In contrast, VgluT3 is found in discrete population of non-glutamatergic neurons using other transmitters than glutamate, such as cholinergic interneurons in the dorsal and ventral striatum, subpopulations of GABAergic basket cells in the cortex and hippocampus, and serotoninergic neurons, where it is involved in more subtle modulation of local transmission. Rat VGluT2 is a 12-transmembrane (a.a. 72-92, 126-146, 149-169, 178-198, 217-237, 245-265, 311-331, 350-370, 387-407, 410-430, 444-464, 478-498) protein with 7 cytoplasmic and 6 vacuolar domains, having both its N- and C-terminal tails (a.a. 1-71 and 499-582) exposed at the cytoplasmic side.
Specificity
This polyclonal antibody targets VGluT2 C-terminal end sequence.
Immunogen
Epitope: C-terminus.
KLH-conjugated linear peptide corresponding to the C-terminal sequence of rat VGluT2.
Application
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected VGluT2 in mouse and rat brain tissue sections.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
This Anti-VGluT2 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of VGluT2.
Quality
Evaluated by Western Blotting in rat brain membrane extract.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected VGluT2 in 10 µg of rat brain membrane extract.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected VGluT2 in 10 µg of rat brain membrane extract.
Target description
~56 kDa observed. 64.58/64.56 kDa (rat/mouse) calculated. The smaller than predicted band size is consistent with the reported apparent molecular weight (Zhang, S., et al. (2015). Nat. Neurosci. 18(3):386-392; Moechars, D., et al. (2006). J. Neurosci. 26(46):12055-12066). Uncharacterized band(s) may appear in some lysates.
Physical form
Guinea pig polyclonal antibody serum with 0.05% sodium azide.
Unpurified.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Nicole Zarate et al.
International journal of molecular sciences, 22(23) (2021-12-11)
PSD-95 (Dlg4) is an ionotropic glutamate receptor scaffolding protein essential in synapse stability and neurotransmission. PSD-95 levels are reduced during aging and in neurodegenerative diseases like Huntington's disease (HD), and it is believed to contribute to synaptic dysfunction and behavioral
Christian Stald Skoven et al.
Frontiers in neuroscience, 16, 968839-968839 (2022-10-11)
Efficient interhemispheric integration of neural activity between left and right primary motor cortex (M1) is critical for inter-limb motor control. We employed optogenetic stimulation to establish a framework for probing transcallosal M1-M1 interactions in rats. We performed optogenetic stimulation of
Yohan S S Auguste et al.
Nature neuroscience, 25(10), 1273-1278 (2022-09-29)
Oligodendrocyte precursor cells (OPCs) give rise to myelinating oligodendrocytes throughout life, but the functions of OPCs are not limited to oligodendrogenesis. Here we show that OPCs contribute to thalamocortical presynapse elimination in the developing and adult mouse visual cortex. OPC-mediated
Laura Modol et al.
Neuron, 105(1), 93-105 (2019-11-30)
The developmental journey of cortical interneurons encounters several activity-dependent milestones. During the early postnatal period in developing mice, GABAergic neurons are transient preferential recipients of thalamic inputs and undergo activity-dependent migration arrest, wiring, and programmed cell-death. Despite their importance for
David G Litvin et al.
Brain, behavior, and immunity, 87, 610-633 (2020-02-26)
The pathways for peripheral-to-central immune communication (P → C I-comm) following sterile lung injury (SLI) are unknown. SLI evokes systemic and central inflammation, which alters central respiratory control and viscerosensory transmission in the nucleus tractus solitarii (nTS). These functional changes coincide with
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service