AB15894
Anti-Tbr2 Antibody
from chicken, purified by affinity chromatography
Synonym(s):
Eomesodermin homolog, T-box brain protein 2, T-brain-2, TBR-2, Tbr2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
chicken
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
species reactivity (predicted by homology)
human, rat
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgY
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... EOMES(8320)
General description
Eomesodermin homolog (UniProt: O54839; also known as T-box brain protein 2, T-brain-2, TBR-2, Tbr2) is encoded by the Eomes (also known as Tbr2) gene (Gene ID: 13813) in murine species. T-box genes are a family of transcription factors have a highly conserved DNA binding domain named the T-domain. Tbr2 functions as a transcriptional activator that plays a crucial role during gastrulation, mesodermal specification, and limb patterning. It plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. It is also shown to be involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Its DNA-binding region is localized to amino acids 278-458. Conditional inactivation of Tbr2 during early brain development is known to cause microcephaly and severe behavior deficits. Two isoforms of Tbr2 have been described that are produced by alternative splicing. Isoform 2 lacks amino acids 463-481. (Ref.: Bulfone, A., et al. (1999). Mech. Dev. 84 (1-2); 133-138).
Specificity
This chicken polyclonal antibody specifically detects T-box brain protein 2 (Tbr2). It targets an epitope within 20 amino acids from the C-terminal region.
Immunogen
Epitope: Transcription Activation Domain
KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of mouse T-box brain protein 2 (Tbr2).
Application
Anti-Tbr2, Cat. No. AB15894, is a chicken polyclonal antibody that detects Tbr2 and is tested for use in Western Blotting and Immunohistochemistry (Paraffin).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Tested Applications
- Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Tbr2 in Mouse brain tissue sections.
- Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Quality
Evaluated by Western Blotting in E13-E14 Mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tbr2 in E13-E14 Mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tbr2 in E13-E14 Mouse brain tissue lysate.
Target description
~72 and 74 kDa observed; 74.8 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity purified
Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommended storage: +2°C to +8°C.
Analysis Note
Control
E13-14 mouse brain lysate
E13-14 mouse brain lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Marta Florio et al.
Science advances, 2(12), e1601941-e1601941 (2016-12-14)
The gene ARHGAP11B promotes basal progenitor amplification and is implicated in neocortex expansion. It arose on the human evolutionary lineage by partial duplication of ARHGAP11A, which encodes a Rho guanosine triphosphatase-activating protein (RhoGAP). However, a lack of 55 nucleotides in
Mareike Albert et al.
The EMBO journal, 36(17), 2642-2658 (2017-08-03)
The generation of neocortical neurons from neural progenitor cells (NPCs) is primarily controlled by transcription factors binding to DNA in the context of chromatin. To understand the complex layer of regulation that orchestrates different NPC types from the same DNA
Natalie E Patzlaff et al.
Human molecular genetics, 26(7), 1340-1352 (2017-02-17)
Fragile X related protein 1 (FXR1P) is a member of the fragile X family of RNA-binding proteins, which includes FMRP and FXR2P. Both FMRP and FXR2P regulate neurogenesis, a process affected in a number of neurological and neuropsychiatric disorders, including
Anna Gorelik et al.
Nature communications, 8, 15096-15096 (2017-05-04)
In recent years the notion that malfunctioning of the immune system may result in developmental brain diseases has emerged. However, the role of immune molecules in the developing brain has not been well explored. The complement pathway converges to cleave
Elisa Penna et al.
Cerebral cortex communications, 2(4), tgab053-tgab053 (2021-10-15)
Microglial cells, the innate immune cells of the brain, are derived from yolk sac precursor cells, begin to colonize the telencephalon at the onset of cortical neurogenesis, and occupy specific layers including the telencephalic proliferative zones. Microglia are an intrinsic
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