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General description
The pCDF-2 Ek/LIC Vector is a LIC-compatible derivative of pCDF-1b. The plasmid contains a strong T7lac promoter and an amino terminal His•Tag coding sequence immediately followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional C-terminal S•Tag coding sequence is compatible with purification, detection, and quantification (1). This plasmid is compatible with the LIC Duet Adaptors for coexpression of two proteins as well as with pET-46 and pRSF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors.
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Components
•: 1 µg: Ek/LIC Vector
•: 25 units: T4 DNA Polymerase, LIC-qualified
•: 50 µl: 25 mM EDTA
•: 40 µl: 25 mM dATP
•: 8 µl: Ek/LIC β-gal Control Insert
•: 100 µl: 100 mM DTT
•: 50 µl: 10X T4 DNA Polymerase Buffer
•: 1.5 ml: Nuclease-free Water
•: 5 × 2 ml: SOC Medium
•: 10 µl: Test Plasmid
•: 0.2 ml: BL21(DE3)pLysS Competent Cells
•: 0.2 ml: BL21(DE3) Competent Cells
•: 22 × 50 µl: NovaBlue GigaSingles Competent Cells
•: 25 units: T4 DNA Polymerase, LIC-qualified
•: 50 µl: 25 mM EDTA
•: 40 µl: 25 mM dATP
•: 8 µl: Ek/LIC β-gal Control Insert
•: 100 µl: 100 mM DTT
•: 50 µl: 10X T4 DNA Polymerase Buffer
•: 1.5 ml: Nuclease-free Water
•: 5 × 2 ml: SOC Medium
•: 10 µl: Test Plasmid
•: 0.2 ml: BL21(DE3)pLysS Competent Cells
•: 0.2 ml: BL21(DE3) Competent Cells
•: 22 × 50 µl: NovaBlue GigaSingles Competent Cells
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Other Notes
1. Kim, J.S. and Raines, R.T. (1993) Protein Science2, 348–356.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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