71012

HT96 BL21(DE3) Competent Cells - Novagen

Escherichia coli, rod shaped

• Synonym(s): BL21 strain
• UNSPSC Code: 41106202
• NACRES: NA.81

Properties

• product name: HT96 BL21(DE3) Competent Cells - Novagen, The HT96 BL21(DE3) Competent Cells are designed for high-throughput transformation and protein expression applications.
• biological source: Escherichia coli
• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: transformation efficiency: >2×10⁶ cfu/μg
• shipped in: dry ice
• storage temp.: 2-8°C

Description

General description

Genotype: F^-ompT hsdS_B(r_B^- m_B^-) gal dcm (DE3)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

The HT96 BL21(DE3) Competent Cells are designed for high-throughput transformation and protein expression applications.

The HT96<TMSYMBOL></TMSYMBOL> BL21(DE3) Competent Cells aredesigned for high-throughput transformation.The cells are predispensed in 20-l volumesin a sturdy 96-well polypropylene plate,which is compatible with a variety of thermalcyclers and water baths. Wells are individuallysealed and have raised rims to prevent cross-contamination.Seals can be easily piercedwith standard pipet tips or removed for access.Strips of caps are also provided for reliablesealing during manipulation and storage.Groups of 24 wells can be easily split fromthe plate for processing smaller numbers of samples.




HT96 BL21(DE3) Competent Cells
BL21 is the most widely used host background and has the advantage of being deficient in both lon (1) and ompT proteases. The designation (DE3) indicates that the strain is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter.

Features and Benefits

• High-throughput, 96-well format

Components

1 plate4 plates20 plates

•1 plate4 plates20 platesHT96 BL21(DE3) Competent Cells

•1 × 14 ml4 × 14 ml20 × 14 mlSOC Medium

•1 × 10 µl2 × 10 µl10 × 10 µlTest Plasmid

•1 pkg4 pkg20 pkg8-Cap Strips

•1420Reagent Reservoirs

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Other Notes

Phillips, T.A., et al. 1984. J. Bacteriol.159, 283.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable