506170

p53-Snail binding Inhibitor, GN25

The p53-Snail binding Inhibitor, GN25 controls the biological activity of p53-Snail. This small molecule/inhibitor is primarily used for Neuroscience applications.

• Synonym(s): p53-Snail binding Inhibitor, GN25, GN25
• Empirical Formula (Hill Notation): C₁₅H₁₄O₆S
• Molecular Weight: 322.33
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: orange
• solubility: DMSO: 50 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A cell-permeable, water soluble and specific p53-Snail binding inhibitor that activates p53 in a K-Ras-dependent manner and displays anti-proliferating effect toward K-Ras-MEF (K-Ras-transformed mouse embryonic fibroblast) cells with 20% viability at 10µM, but not in N-Ras/Myc-MEF or non-transformed MEF. Snail and p53 expression are elevated in a dose dependent manner after GN25 treatment at 1-10µM in K-Ras-mutated human cancer cell lines A549, but not in MKN-45 (wt-K-Ras/wt-p53) cells. Furthermore, it is shown to selectively activate wild-type p53 in p53WT/MT cancer cells, but not Panc-1 cells with single allelic mutant p53. Exhibits anti-tumor effect in a xenograft model in vivo.

A cell-permeable, water soluble and specific p53-Snail binding inhibitor that activates p53 in a K-Ras-dependent manner and displays anti-proliferating effect toward K-Ras-MEF (K-Ras-transformed mouse embryonic fibroblast) cells with 20% viability at 10µM, but not in N-Ras/Myc-MEF or non-transformed MEF. Snail and p53 expression are elevated in a dose dependent manner after GN25 treatment at 1-10µM in K-Ras-mutated human cancer cell lines A549, but not in MKN-45 (wt-K-Ras/wt-p53) cells. Furthermore, it is shown to selectively activate wild-type p53 in p53WT/MT cancer cells, but not Panc-1 cells with single allelic mutant p53. Exhibits anti-tumor effect in a xenograft model in vivo.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Lee, S., et al. 2010. Oncogene29, 4576.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable