1.52007
Chromolith® RP-18 endcapped (monolithic) HPLC Columns
L × I.D. 50 mm x 2 mm HPLC Column
Synonym(s):
Chromolith® FastGradient RP-18 endcapped 50-2 HPLC column
About This Item
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product name
Chromolith® RP-18 endcapped, L × I.D. 50 mm x 2 mm HPLC column
material
PEEK column
Quality Level
Agency
suitable for USP L1
product line
Chromolith®
feature
endcapped
packaging
1 ea of
extent of labeling
18% Carbon loading
parameter
200 bar pressure
45 °C max. temp.
technique(s)
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
5 cm × 2 mm
matrix
monolithic silica
matrix active group
C18 bonding phase
pore size
1.5 μm macroporosity
13 nm mesoporosity
operating pH range
2-7.5
separation technique
reversed phase
storage temp.
no temp limit
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General description
Chromolith® FastGradient RP-18 endcapped 50-2 columns have macropores with 1.5 μm diameter, giving a column efficiency exceeding 100,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® FastGradient RP-18 endcapped 50-2 columns fall under the USP L1 Classification. The column efficiency is comparable to a 3-4 μm particle size fully porous particulate column. Chromolith® FastGradient RP-18 endcapped 50-2 columns are perfectly suitable for LC-MS applications.
The column dimension of 50 mm length and 2 mm I.D. is the best choice for fast separations on any U/HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enable:
- Rapid separations at very low column back-pressure
- High matrix tolerance, allowing time and cost savings with simplified workflows
- Cost-savings due to extended column lifetimes
- Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-2 guard cartridges (3 pieces) [1.52009] with the Chromolith® Guard Cartridge Holder (kit) 5 mm x 2 mm [1.52004].
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467].
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Analysis Note
Pressure: ≤ 30 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 100000
Capacity factor (Anthracene): 3.6 - 4.3
Tailing (USP): 0.86 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 2.30 - 2.60
Legal Information
Certificates of Analysis (COA)
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Articles
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.
Related Content
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).
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