1.24542

Nicotinamide adenine dinucleotide free acid

for biochemistry NAD

• Synonym(s): Nicotinamide adenine dinucleotide free acid, NAD free acid, DPN
• Empirical Formula (Hill Notation): C₂₁H₂₇N₇O₁₄P₂
• CAS Number: 53-84-9
• Molecular Weight: 663.43
• MDL number: MFCD00036253
• UNSPSC Code: 12352107
• EC Index Number: 200-184-4
• NACRES: NB.21

Properties

• Quality Level: 200
• form: solid
• pH: 2.5-3.0 (20 °C, 10 g/L in H₂O)
• mp: 140-142 °C
• solubility: 10 g/L
• bulk density: 200‑300 kg/m³
• storage temp.: 2-8°C
• InChI: 1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)
• InChI key: BAWFJGJZGIEFAR-UHFFFAOYSA-N

Description

Application

• Development of a Highly Selective NAD(+) -Dependent Glyceraldehyde Dehydrogenase and its Application in Minimal Cell-Free Enzyme Cascades: This research presents the development of an NAD+-dependent enzyme crucial for bioindustrial applications, demonstrating its utility in enzyme cascades for biosynthesis processes, which could enhance the efficiency of biotechnological production systems (DOI: 10.1002/css).

Analysis Note

Assay (β-NAD, enzymatically with ADH; calc. on anhydrous substance): ≥ 95 %
Assay (spectrophotometrically): ≥ 95.0 %
Identity (IR-spectrum): passes test
Appearance: White to almost white, fine powder eventuel slightly yellowish.
pH (10 g/l, water): 2.5 - 3.0
Absorption maximum λ_max. (buffer pH 7.0): 259 - 261 nm
Spec. Absorptivity A1%/1cm (λ_max.; 0,01 g/l; buffer pH 7.0; calc. on anhydrous substance): 255 - 270
Water (according to Karl Fischer): ≤ 7.0 %

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable