07-582-I

Anti-phospho-IRF-3 (Ser396) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Interferon regulatory factor 3, IRF-3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_001562
• UniProt accession no.: Q14653
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer396)
• Gene Information: human ... IRF3(3661) ; pig ... Irf3(396656) ; rhesus monkey ... Irf3(718970)

Description

General description

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.

Specificity

This antibody recognizes IRF-3 phosphorylated at Ser396.

Immunogen

Epitope: SNS → ANA: Mutation of this residue results in loss of viral-induced phosphorylation of IRF-3.

KLH-conjugated linear peptide corresponding to human IRF-3 phosphorylated at Ser396.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3.

Quality

Evaluated by Western Blot in untreated and sendai virus treated A549 cell lysates.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-IRF-3 (Ser396) in 10 µg of sendai virus treated A549 cell lysate.

Target description

~50 kDa observed. Uncharacterized bands may appear at ~40 kDa and ~80 kDa in some lysates.

Linkage

Replaces: 07-582

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Untreated and sendai virus treated A549 cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable