05-1059

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8, rabbit monoclonal

culture supernatant, clone 8.78.8, from rabbit

• Synonym(s): CSAID-binding protein, Csaids binding protein, Cytokine suppressive anti-inflammatory drug-binding protein, MAP kinase MXI2, MAP kinase p38 alpha, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, cytokine suppressive anti-inflammato
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: 8.78.8, monoclonal
• species reactivity: human, vertebrates, mouse
• technique(s): immunofluorescence: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_001306
• UniProt accession no.: Q15759
• shipped in: wet ice
• target post-translational modification: phosphorylation (pThr180/pTyr182)
• Gene Information: human ... MAPK14(1432)

Description

General description

The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.

Specificity

Predicted to cross-react with many other species based on 100% sequence homology with immunogen.

This antibody detects p38α when phosphorylated on Thr180/Tyr182.

Immunogen

Epitope: Phosphorylated Thr180/Tyr182

Synthetic peptide corresponding to amino acids surrounding and including phosphorylated Thr180/Tyr182 of human p38α.

Application

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8 detects level of phospho-p38α(Thr180/Tyr182) & has been published & validated for use in WB & IF.

Research Sub Category
MAP Kinases

Inflammation & Autoimmune Mechanisms

Quality

Evaluated by western blot on anisomycin-treated HeLa cell lysate.
Western Blot Analysis: 1:5,000-1:10,000 dilution of this antibody was used to detect p38 phosphorylated on Thr180/Tyr182 in anisomycin-treated HeLa and NIH/3T3 cell lysate.

Target description

38 kDa

Physical form

Rabbit monoclonal antibody in culture supernatant containing 0.05% sodium azide.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable