HPLC Analysis of Triazine Herbicides in Grass on SUPELCOSIL™ LC-8-DB after SPE using Supelclean™ LC-SCX
CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
sample/matrix
fresh grass clippings spiked with herbicides at 2 ppm
SPE tube/cartridge
Supelclean LC-SCX, 500 mg/3 mL (57018)
condition
1 mL methylene chloride
sample addition
2 mL grass extract. Wash with 2 x 2 mL acetonitrile.
drying
Dry packing for 5 min. under nitrogen purge
washing
Dry packing for 5 min under nitrogen purge; wash with 2 x 2 mL DI water
elution
1.5 mL methanol
eluate post-treatment
dilute to 2 mL with DI water
column
SUPELCOSIL LC-8-DB, 15 cm × 4.6 mm I.D., 5 μm (58347-U) with guard column (59553)
mobile phase
[A] acetonitrile; [B] water; (45:55, A:B)
flow rate
1.5 mL/min
detector
UV, 254 nm
injection
100 μL
Descripción
Aplicación
Sample Pre-treatment:
5 g fresh grass clippings spiked with 2 ppm each herbicide. Add 4 g anhydrous sodium sulfate and 20 mL methylene chloride:acetone (80:20). Shake 20 min and allow mixture to stand 1 min.
Información legal
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Supelclean is a trademark of Sigma-Aldrich Co. LLC