HPLC Analysis of Positional Isomers (Xylenes) on Astec^® CYCLOBOND I 2000

column

CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)

mobile phase

[A] acetonitrile; [B] water; (15:85, A:B)

flow rate

0.8 mL/min

column temp.

55 °C

detector

UV, 230 nm

injection

3 μL

sample

xylene isomers, each compound, 0.1 mg/mL in acetonitrile:water (50:50) (elution order: m, o, p)