HPLC Analysis of Omeprazole Enantiomers on Astec^® CYCLOBOND I 2000 DMP

column

CYCLOBOND I 2000 DMP, 25 cm x 4.6 mm I.D., 5 μm particles (20724AST)

mobile phase

[A] acetonitrile; [B] acetic acid; [C] ammonium hydroxide; (100:0.4:0.1, A:B:C)

flow rate

1 mL/min

column temp.

23 °C

detector

UV, 302 nm

injection

5 μL

sample

1 mg/mL in methanol