HPLC Analysis of Isocyanate Derivatives on Ascentis^® ES-Cyano

column

Ascentis Cyano, 15 cm x 4.6 mm I.D., 5 μm particles (577306-U)

mobile phase

[A] 0.1% ammonium acetate in water; [B] 0.1% ammonium acetate in acetonitrile; (70:30, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

5 μL

sample

20 μg/mL in 80:12:8, water: acetonitrile: dimethylsulfoxide