HPLC Analysis of HSA Fusion Protein on Zenix^®-C SEC-300 vs. Zenix^® SEC-300

column

Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777033)

column

Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777092)

mobile phase

150 mM sodium phosphate buffer, pH 7.0

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 280 nm

injection

10 μL

sample

HSA fusion peptide 5 mg/mL (MW 75 kD, pl 5.0, HSA conjugated peptide in diabetes treatment)