HPLC Analysis of Carotenes on Ascentis^® Express RP-Amide, Methanol Mobile Phase

column

Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 μm particles (53931-U)

mobile phase

methanol

flow rate

1 mL/min

pressure

1653 psi (114 bar)

column temp.

30 °C

detector

UV-VIS, 454 nm

injection

5 μL

sample

V8 Splash^® fruit juice was extracted with hexane, the extract dissolved 1:1 into ethanol for HPLC injection