HPLC Analysis of Benzoic Acid and Related Compounds on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm × 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

[A] water with 0.1% TFA; [B] acetonitrile; (70:30, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 220 nm

injection

10 μL