HPLC Analysis of Benzodiazepines on Ascentis^® Express C18, Acetonitrile Mobile Phase

column

Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 μm particles (53814-U)

mobile phase

[A] water; [B] acetonitrile; (66:34, A:B)

flow rate

0.6 mL/min

column temp.

35 °C

detector

UV, 250 nm

injection

2 μL

sample

0.1 g/L each in 20% methanol