HPLC Analysis of Acrylamide in Fried Potato Chips on Discovery® HS F5 after SPE using Discovery® DSC-MCAX and DSC-18
Materiales
columna analítica
CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
sample/matrix
Finely grind 2.0 g of potato chips, place in 20 mL vial and add 10 mL water. Mix using vortex until mixture forms a thick paste. (Place sample in centrifuge tubes and centrifuge at 16.1 rcf for 5 min. Extract aqueous portion of sample leaving oil layer and solids.)
SPE tube/cartridge
Discovery DSC-MCAX, 300 mg/3 mL (52784-U)
SPE tube/cartridge
Discovery DSC-18, 1g/6mL (52606-U)
condition
connect a Discovery DSC-MCAX SPE tube to the top of a Discovery DSC-18 SPE tube using an SPE tube adapter, pass 1 mL methanol, then 1 mL DI water through both SPE tubes until the tubes are dry
sample addition
1 mL of aqueous extract
washing
1 mL DI water
elution
removing upper MCAX SPE tube, pass 2 mL methanol through lower DSC-18 SPE tube
eluate post-treatment
evaporate to dryness with nitrogen at 30 °C, reconstitute in 0.5 mL DI water
column
Discovery HS F5, 15 cm x 4.6 mm I.D., 3 μm particles (567507-U)
mobile phase
100% ultra-pure water
column temp.
35 °C
detector
ESI+
injection
5 μL
Descripción
Información legal
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany