GC Analysis of FAMEs in Fish Oil Capsules on Omegawax
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CONDITIONS
column
Omegawax, 30 m x 0.25 mm I.D., 0.25μm (24136)
oven
170 °C, 1 °C/min to 225°C
inj. temp.
250 °C
detector
FID 260 °C
carrier gas
helium, 1.2 mL/min
injection
1 μL, 100:1 split
liner
4 mm ID FocusLiner with taper
sample
1. Fish Oil from capsule, prepared according to AOAC Method 991.39 2. Atlantic, farm-raised salmon, prepared according to AOCS Official Method Ce 1k-09
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Nota de análisis
Samples of a commercially purchased fish oil capsule were analyzed for fatty acids. Sample preparation of the fish oil capsule was done as described in AOAC Official Method 991.39 (1). The resulting extracts were then analyzed by GC/FID on an Omegawax 250 capillary column as described in AOCS Official Method Ce 1i-07.
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