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Merck

Human adipose tissue-derived mesenchymal stromal cells promote B-cell motility and chemoattraction.

Cytotherapy (2014-09-23)
Laura Barrio, Victor Delgado Cuevas, Ramón Menta, Pablo Mancheño-Corvo, Olga delaRosa, Wilfried Dalemans, Eleuterio Lombardo, Yolanda R Carrasco
RESUMEN

Mesenchymal stromal cells hold special interest for cell-based therapy because of their tissue-regenerative and immunosuppressive abilities. B-cell involvement in chronic inflammatory and autoimmune pathologies makes them a desirable target for cell-based therapy. Mesenchymal stromal cells are able to regulate B-cell function; although the mechanisms are little known, they imply cell-to-cell contact. We studied the ability of human adipose tissue-derived mesenchymal stromal cells (ASCs) to attract B cells. We show that ASCs promote B-cell migration through the secretion of chemotactic factors. Inflammatory/innate signals do not modify ASC capacity to mediate B-cell motility and chemotaxis. Analysis of a panel of B cell-related chemokines showed that none of them appeared to be responsible for B-cell motility. Other ASC-secreted factors able to promote cell motility and chemotaxis, such as the cytokine interleukin-8 and prostaglandin E2, did not appear to be implicated. We propose that ASC promotion of B-cell migration by undefined secreted factors is crucial for ASC regulation of B-cell responses.

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Prostaglandin E2, synthetic, powder, BioReagent, suitable for cell culture
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Prostaglandin E2, γ-irradiated, powder, BioXtra, suitable for cell culture
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Prostaglandin E2, ≥93% (HPLC), synthetic
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Dinoprostone, European Pharmacopoeia (EP) Reference Standard
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