G001635

HPLC Analysis of 3-Methylpyrazole and 4-Methylpyrazole in Urine on Discovery^® C18 after SPE using Discovery^® DSC-SCX

application for SPE, application for HPLC

Propiedades

• técnicas: HPLC: suitable; solid phase extraction (SPE): suitable
• parámetros analíticos: sample preparation: SPE (Solid Phase Extraction); sample/matrix: urine spiked with 3- and 4-methylpyrazole at 1 mg/mL; SPE tube/cartridge: Discovery DSC-SCX, 500 mg/3 mL (52686-U); condition: 2 mL methanol; 2 mL DI water; sample addition: 1 mL; washing: 2 mL DI water; elution: 2 mL 5% methanol in 250 mM potassium phosphate buffer, pH 7.4; column: Discovery C18, 15 cm × 4.6 mm, 5 μm preceded by a 2 cm guard column and 0.5 μm frit filter (504955); mobile phase: MeOH:5 mM phosphate buffer, pH 6 (20:80); flow rate: 1 mL/min; column temp.: 30 °C; detector: UV, 220 nm; injection: 25 μL, dilute urine extract
• idoneidad: application for HPLC; application for SPE
• aplicaciones: forensics and toxicology

Descripción

Información legal

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analito

3-Methylpyrazole