823426

TOYOPEARL^® AF-rProtein A HC-650F Bulk Media

phase rProtein A, bottle of 25 mL, 45 μm particle size

• Sinónimos: TOYOPEARL^® AF-rProtein A HC-650F Bulk Media
• Código UNSPSC: 41106500

Propiedades

• descripción: Adsorption capacity (> 80 g/L IgG (static); >70 g/L IgG (dynamic at 5 min. residence time)); Phase filter (Protein A)
• Línea del producto: TOYOPEARL^®
• formulario: slurry
• envase: bottle of 25 mL
• fabricante / nombre comercial: Tosoh 23426
• Parámetros: 3 bar max. pressure
• técnicas: LPLC: suitable
• matriz: hydroxylated methacrylic polymer particle platform
• grupo activo de la matriz: rProtein A phase
• tamaño de partícula: 45 μm
• tamaño de poro: 1000 Å
• cleaning in place: 0.1 M NaOH
• pH: 2-13
• capacidad: >70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time); >80 g/L adsorption capacity (IgG)(static)
• técnica de separación: affinity
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

TOYOPEARL AF-rProtein A HC-650F is a high capacity Protein A resin for monoclonal antibody purification. An enhanced rProtein A ligand is bound to the TOYOPEARL HW-65F base bead via multipoint attachment resulting in excellent base stability for up to 200 CIP cycles with 0.1 mol/L NaOH.
TOYOPEARL AF-rProtein A HC-650F resin maintains 80% of initial dynamic binding capacity after 40 CIP cycles with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A HC-650F resin exhibits dynamic binding capacities of greater than 65 g/L at residence times of 5 minutes and greater than 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L.
Achievement of high linear velocities at relatively low pressure enables high throughput at production scale using equipment with moderate pressure limitations. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to agarose base stable resins. Typical leakage for this rProtein A ligand is 0.6 -1.7 ng rProtein A /mg eluted antibody by ELISA testing. Though TOYOPEARL AF-rProtein A-650F resin is shipped in 20% ethanol, it can be stored in 2% benzyl alcohol if necessary.
Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at low residence times.

Especificaciones

Clean in place with 0.1 M NaOH.

Forma física

72% (v/v) slurry in 20% (v/v) ethanol

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Información legal

Toyopearl is a registered trademark of Tosoh Corporation

Información de seguridad

• Pictogramas: GHS02
• Palabra de señalización: Warning
• Frases de peligro: H226
• Consejos de prudencia: P210 - P233 - P240 - P241 - P242 - P243
• Clasificaciones de peligro: Flam. Liq. 3
• Código de clase de almacenamiento: 3 - Flammable liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): 104.0 °F - closed cup
• Punto de inflamabilidad (°C): 40 °C - closed cup