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Merck

575659-U

Supelco

HybridSPE®-PLus 96-Well solid phase extraction (SPE) Plate

Volume 2 mL, bed wt., 50 mg, pk of 1 ea

Sinónimos:

HybridSPE®-PLus

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About This Item

Código UNSPSC:
41104018
NACRES:
NB.21

product name

HybridSPE®-PLus 96-Well Plate, volume 2 mL, pk of 1 ea

descripción

96-well Plate

Nivel de calidad

Línea del producto

HybridSPE®

composición

bed wt., 50 mg

envase

pk of 1 ea

técnicas

solid phase extraction (SPE): suitable

volumen

2 mL

grupo activo de la matriz

zirconia-based phase

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Descripción general

HybridSPE-PLus is the next generation of HybridSPE-Phospholipid technology. The first of its kind, HybridSPE-Phospholipid technology was first introduced in 2008. It fuses the simple, standardized methodology of traditional protein precipitation with the specificity of solid phase extraction (SPE) for the simultaneous removal of proteins and phospholipids from biological samples prior to LC-MS analysis. Unlike other phospholipid removal products that use a hydrophobic retention mechanism to remove phsopholipids from biological samples, HybridSPE-Phospholipid technology uses a unique Lewis acid/base retention mechanism that allows it to separate phospholipids from even very hydrophobic analytes, such as vitamins, which are often retained along with phospholipids on competitive products.

Our HybridSPE-PLus products offer the same time-proven chemistry of our traditional HybridSPE-Phospholipid products coupled with manufacturing enhancements that stem from years of production experience. These manufacturing enhancements have allowed us to increase flow consistency and reduce sample hold up volumes, further improving analyte recoveries and assay reproducibility. We invite customers to take advantage of the newest technology; HybridSPE-PLus.

Características y beneficios

  • Simple 2-3 step standard methodology analogous to traditional protein precipitation provides simultaneous removal of both proteins and phospholipids
  • Unique Lewis acid/base retention mechanism effectively removes phospholipids from both hydrophilic and hydrophobic analytes of interest (unlike competitive chemistries) eliminating phospholipid-induced ion suppression
  • Highly reproducible, consistent product performance
  • Provides cleaner samples for longer column life and less instrument downtime
  • Available in 96-well and 1 mL cartridge dimensions

Información legal

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Certificados de análisis (COA)

Lot/Batch Number

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Artículos

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.

Using HybridSPE-Phospholipid, Ascentis Express, and Other LC-MS Workflow Components

Protocolos

Immunoassay-based methods for digitoxin or digoxin exist, but are time consuming, labor intensive, and not selective due to similarities in their chemical structures.

LC/MS/MS Analysis of Interacting Cardiac Drugs Digoxin, Quinidine, Amiodarone and Verapamil on Titan™ C18

Contenido relacionado

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

Solid phase extraction purifies samples for chromatographic analysis, improving data quality and protecting analytical columns.

Chromatograms

application for HPLC, application for SPE

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