ZRB2344

Anti-CD3ζ/CD247 Antibody, clone 1N11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: CD247, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1N11, recombinant monoclonal
• descripción: 1N11 Clone
• Línea del producto: ZooMAb^® learn more
• Formulario: lyophilized
• mol peso: calculated mol wt 18.7 kDa; observed mol wt ~16 kDa
• purificado por: using Protein A
• reactividad de especies: human
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• técnicas: affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: C-terminal half
• Nº de acceso Protein ID: NP_932170
• Nº de acceso UniProt: P20963
• categoría alternativa más sostenible: Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: human ... CD247(919)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 1N11 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects CD3 / CD247. It targets an epitope within 22 amino acids from the C-terminal half.

Inmunógeno

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human CD3 /CD247.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CD3 /CD247 in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CD3 /CD247 in Human spleen tissue lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD3 /CD247 in Jurkat cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CD3 /CD247 in human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound CD3 with a KD of 5.0 x 10-7 in an affinity binding assay.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected CD3 /CD247 in one million Jurkat cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

T-cell surface glycoprotein CD3 zeta chain (UniProt: P20963; also known as T-cell receptor T3 zeta chain, CD247) is encoded by the CD247 (also known as CD3Z, T3Z, TCRZ) gene (Gene ID: 919) in human. CD3 ζ  is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains a short extracellular domain (aa 22-30), a transmembrane domain (aa 31-51), and a cytoplasmic domain (aa 52-164).  CD3ζ is expressed in normal lymphoid tissues and in peripheral blood mononuclear cells (PBMC). It is a part of the TCR-CD3 complex that is present of the surface of T-cells and plays an essential role in adaptive immune response.  When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by various CD3 chains (CD3D, CD3E, CD3G and CD3Z). These chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. The ITAM motifs CD3ζ are localized to amino acids 61-89; 100-128; and 131-159. These motifs are reported to be phosphorylated by Src family protein tyrosine kinases Lck and Fyn, resulting in the activation of downstream signaling pathways. In CD3 ζ phosphorylation of ITAMs by Lck is reported to be an initial step in the transduction of signaling cascades. It creates multiple docking sites for protein kinase ZAP70, which is also phosphorylated by Lck. Phosphorylation of ZAP70 converts it into a catalytically active form. It is reported that CD3ζ phosphorylation can also be promoted by ZAP70 and TCR-induced zeta phosphorylation is shown to be defective in ZAP70-deficient T-cells. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Iwashima, M., et al. (1994). Science. 263(5150); 1136-1139; Barber, EK., et al. (1989). Proc. Natl. Acad. Sci. USA. 86(9); 3277-3281).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitución

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable