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Key Documents

ZRB2289

Sigma-Aldrich

Anti-Notch 3 Antibody, clone 1G17 ZooMAb® Rabbit Monoclonal

enhanced validation

recombinant, expressed in HEK 293 cells

Sinónimos:

Neurogenic locus notch homolog protein 3

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

origen biológico

rabbit

Nivel de calidad

recombinante

expressed in HEK 293 cells

conjugado

unconjugated

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

1G17, recombinant monoclonal

descripción

recombinant, expressed in HEK 293 cells

Línea del producto

ZooMAb® learn more

formulario

lyophilized

purificado por

using Protein A

reactividad de especies

mouse, human

reactividad de especies (predicha por homología)

rat

envase

antibody small pack of 25 μL

características de los productos alternativos más sostenibles

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

validación mejorada

recombinant expression
Learn more about Antibody Enhanced Validation

técnicas

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotipo

IgG

secuencia del epítopo

C-terminal

Nº de acceso Protein ID

Nº de acceso UniProt

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

Información sobre el gen

human ... NOTCH3(4854)

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 1G17 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Notch 3. It targets an epitope within 17 amino acids from the C-terminal region.

Inmunógeno

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Notch 3.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Notch 3 in K562 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Notch 3 in MCF-7, HepG2, and Neuro2a cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound Notch 3 peptide with a KD of 2.2 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Notch 3 in MCF-7 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Notch 3 in Human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Neurogenic locus notch homolog protein 3 (UniProt: Q9UM47; also known as Notch 3) is encoded by the NOTCH3 gene (Gene ID: 4854) in human. Notch 3 is a single-pass, type I membrane protein that is synthesized with a signal peptide (aa 1-39), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 40-1643), a transmembrane domain (aa 1644-1664), and a cytoplasmic domain (aa 1665-2321). It is ubiquitously expressed in fetal and adult tissues. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination. Notch3 is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in the formation of a C-terminal fragment and an N-terminal fragment. Mature Notch3 assembles as a heterodimeric protein. Binding of ligand triggers heterodimer dissociation that exposes proteolytic cleavage site (aa 1571-1572) resulting in the release of the Notch intracellular domain (NICD) that translocates to the nucleus and activates transcription of downstream target genes. Its N-terminal half contains 34 EGF-like domains, of which domains 10 and 11 are required for binding to its ligands. The C-terminal half contains 5 Ankyrin repeats. Mutations in the NOTCH3 gene have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Pathogenic mutations predominantly affect cysteine residues within EGF-like repeats in the extracellular domain. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Peters, N., et al. (2004). EXP®. Cell Res. 299(2); 454-464; Arboleda-Velasquez, JF., et al. (2002). Neurology. 59(2); 277-279; Dichgans, M., et al. (2000). Eur. J. Hum. Genet. 8(4); 280-285).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitución

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

Affinity is a trademark of Mine Safety Appliances Co.
EXP is a registered trademark of Optimize Technologies, Inc.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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