ZRB2166

Anti-SIGLEC15 Antibody, clone 1B10 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Sialic acid-binding Ig-like lectin 15;Siglec-15;CD33 antigen-like 3
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1B10, recombinant monoclonal
• descripción: 1B10 Clone
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 35.65 kDa; observed mol wt ~38 kDa
• purificado por: using Protein A
• reactividad de especies: human
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: C-terminal cytoplasmic domain
• Nº de acceso Protein ID: NP_998767
• Nº de acceso UniProt: Q6ZMC9
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SIGLEC15(284266)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 1B10 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Sialic acid-binding Ig-like lectin 15 (SIGLEC15). It targets an epitope within 24 amino acids from the C-terminal, cytoplasmic domain.

Inmunógeno

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal, cytoplasmic domain of human Sialic acid-binding Ig-like lectin 15 (SIGLEC15).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis (WB): A 1:10,000 Dilution of this antibody detected SIGLEC15 in Raji cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SIGLEC15 in Human Tonsil tissue lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SIGLEC15 in Raji cells.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected SIGLEC15 in one million Raji cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SIGLEC15 in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Descripción de destino

Sialic acid-binding Ig-like lectin 15 (UniProt: Q6ZMC9; also known as Siglec-15, CD33 antigen-like 3) is encoded by the SIGLEC15 (also known as :CD33L3) gene (Gene ID: 284266) in human. SIGLEC15 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 20-263), a transmembrane domain (aa 264-284), and a cytoplasmic domain (aa 295-328). Its expression is observed in macrophages and in dendritic cells of spleen and lymph nodes. It serves as an immune checkpoint molecule and a critical immune suppressor. It suppresses antigen-specific T cell responses in vitro and in vivo. Genetic ablation or antibody blockade of SIGLEC15 is reported to amplify anti-tumor immunity in the tumor microenvironment and inhibits tumor growth in murine models. SIGLEC15-deficient mice also exhibit decreased serum IL-10 levels. Two isoforms of SIGLEC15 are described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang, J., et al. (2019). Nat. Med. 25; 656-666).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1