ZRB2053

Anti-SLC12A2 Antibody, clone 1J6 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Solute carrier family 12 member 2 (UniProt: P55011; also known as Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2
• Código UNSPSC: 12352203

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1J6, recombinant monoclonal
• descripción: 1J6 Clone
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 131.45 kDa; observed mol wt ~130 kDa
• purificado por: using Protein A
• reactividad de especies: human, mouse
• reactividad de especies (predicha por homología): rat, horse, porcine, monkey, feline, bovine, canine
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: C-terminal cytoplasmic domain
• Nº de acceso Protein ID: NP_001037
• Nº de acceso UniProt: P55011
• categoría alternativa más sostenible: , Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SLC12A2(6558)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 1J6 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Solute carrier family 12 member 2 (SLC12A2). It targets an epitope within 19 amino acids from the C-terminal, cytoplasmic domain.

Inmunógeno

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal, cytoplasmic domain of human Solute carrier family 12 member 2 (SLC12A2).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SLC12A2 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected SLC12A2 in Human and Mouse brain tissue lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SLC12A2 in human pancreas tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SLC12A2 in HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Solute carrier family 12 member 2 (UniProt: P55011; also known as Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, SLC12A2) is encoded by the SLC12A2 (also known as NKCC1) gene (Gene ID: 6558) in human. SLC12A2 belongs to the subfamily of cation-chloride cotransporters that mediate electroneutral transport of sodium, potassium, and chloride across the plasma membrane. It is a multi-pass membrane protein with seven cytoplasmic domains, sixteen transmembrane domains (including two discontinuous), and six extracellular domains. It is expressed in many tissues and plays a vital role in the regulation of ionic balance and cell volume. Its activity is inhibited by bumetanide and furosemide-type of compounds. It has a major impact on intracellular chloride ion concentration in vascular smooth muscle cells (VSMC) and in neuronal cells. In primary hypertension, its activity is increased in VSMC and paraventricular nucleus neurons that leads to elevated peripheral resistance in systemic circulation and activation of sympathetic nervous system. Mutations in SLC12A2 gene have been linked to antenatal Bartter syndrome 1, an autosomal recessive disorder that is characterized by impaired salt reabsorption in the thick ascending loop of Henle, salt wasting, hypokalemic metabolic alkalosis, and hypercalcinuria. Some mutations are also associated with neurodevelopment disorders and hearing loss. Two isoforms of SLC12A2 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mutai, H., et al. (2020). PLoS Genet.16(4); e1008643; Stodberg, T., et al. (2020). Neurol Genet 6; :e478; Simon, DB., et al. (1996). Nat. Genet. 13(2); 183-188).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitución

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable