ZRB1978

Anti-γ Catenin Antibody, clone 1H22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Junction plakoglobin;Desmoplakin III;Desmoplakin-3
• Código UNSPSC: 12352203

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1H22, recombinant monoclonal
• descripción: 1H22 Clone
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 81.75 kDa; observed mol wt ~80 kDa
• purificado por: using Protein A
• reactividad de especies: human
• reactividad de especies (predicha por homología): monkey
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• técnicas: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: N-terminus
• Nº de acceso Protein ID: NP_002221
• Nº de acceso UniProt: P14923
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... JUP(3728)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 1H22 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Catenin. It targets an epitope within 23 amino acids from the N-terminal region.

Inmunógeno

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal region of human Catenin.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Catenin in A431 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Catenin in MCF-7 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Catenin in MCF-7 cells.

Affinity Binding Assay: A representative lot of this antibody bound Catenin peptide with a KD of 6.5 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Catenin in human colon tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Catenin in A431 cell lysate.

Descripción de destino

Junction plakoglobin (UniProt: P14923; also known as Catenin gamma, Desmoplakin III, Desmoplakin-3) is encoded by the JUP (also known as CTNNG, DP3) gene (Gene ID: 3728) in human. -catenin and β-catenin are homologous proteins that share characteristic structural features with a central armadillo repeat domain flanked by the carboxyl and amino terminal domains. They function in cell-cell junctions when coupled with cadherins. -catenin is a homodimeric protein that is a component of an E-cadherin/catenin adhesion complex. It is present in adherens junctions and desmosomes where it interacts with desmoglein and desmocollin. The presence of -catenin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. In β-catenin knockout (KO) mice -catenin is reported to compensate for β-catenin loss at adherens junction without affecting desmosomes but it is unable to fulfill functions in Wnt signaling. It can also bind to LEF-1 and, when overexpressed in mammalian cells, enhances LEF-1-directed transcription. However, the -catenin and LEF-1 complex may not be efficient in binding DNA or in forming a ternary complex containing LEF-1 and the LEF-1-binding DNA sequences. Overexpression of -catenin does not directly activate transcription but may induce transcription indirectly by elevating the level of endogenous β-catenin by blocking degradation of β-catenin in the cytoplasm. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wickline, ED., et al. (2013). Neoplasia.15(4); 421- 434; Maeda, O., et al. (2004). Oncogene. 23(4); 964-972).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable