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ZRB1745

Sigma-Aldrich

Anti-TMEM206 Antibody, clone 2H13 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sinónimos:

Proton-activated chloride channel;PAC;Transmembrane protein 206

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

origen biológico

rabbit

Nivel de calidad

recombinante

expressed in HEK 293 cells

conjugado

unconjugated

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

2H13, recombinant monoclonal

descripción

recombinant, expressed in HEK 293 cells

Línea del producto

ZooMAb® learn more

formulario

lyophilized

mol peso

calculated mol wt 40.04 kDa
observed mol wt ~48 kDa

purificado por

using Protein A

reactividad de especies

mouse, human, rat

envase

antibody small pack of 25 μL

características de los productos alternativos más sostenibles

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

validación mejorada

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

técnicas

ELISA: suitable
affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable

isotipo

IgG

secuencia del epítopo

N-terminal

Nº de acceso Protein ID

Nº de acceso UniProt

categoría alternativa más sostenible

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... PACC1(55248)

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 2H13 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Transmembrane protein 206 (TMEM206). It targets an epitope within 24 amino acids from the N-terminal region.

Inmunógeno

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of human Transmembrane protein 206 (TMEM206).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in Human brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TMEM206 in Human brain tissue lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TMEM206 in lysates from Mouse brain tissue and PC12 cells, and recombinant Human TMEM206 protein.

Enzyme Immunoassay Analysis (ELISA): Serial dilutions from a representative lot detected TMEM206 protein.

Affinity Binding Assay: A representative lot of this antibody bound TMEM206 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TMEM206 in HeLa, A431, and NIH 3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in Human brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TMEM206 in Human brain tissue lysate.

Descripción de destino

Proton-activated chloride channel (UniProt: Q9H813; also known as PAC, Transmembrane protein 206) is encoded by the PACC1 (also known as TMEM206) gene (Gene ID: 55248) in human. Chloride ions (Cl ) are the most abundant anions in animal cells and their movement across cell membrane is mediated by Cl channels and transporters, which are essential for several cellular functions, such as regulation of cell volume, acidification of intracellular vesicles, and excitability control in muscle cell. TMEM206 is an evolutionarily conserved, multi-pass membrane glycoprotein with two cytoplasmic domains, two transmembrane domains, and one extracellular domain. It is widely expressed, but its highest expression is observed in the brain. It is an essential component of the acid-sensitive outwardly rectifying (ASOR) anion channel that is involved in import of chloride ions in response to extracellular acidic pH. It is also involved in acidosis-induced cell death by mediating chloride influx and subsequent cell swelling. TMEM206 -/- cells exhibit partial protection from acid-induced cell death. Higher expression of TMEM206 has been observed in colorectal cancer (CRC), breast, and liver cancers. Overexpression of TMEM206 promotes proliferation and invasion in CRC cells and up-regulates the levels of phospho-AKT and its downstream signaling components. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Deng, Z., et al. (2021). Science Adv. 7(9); eabe5983; Zhang, L., et al. (2020). Oncol. Lett. 5; 174; Ullrich, F., et al. (2019). eLife. 8; e49187).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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