ZRB1721

Anti-EGR1 Antibody, clone 1F22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Early growth response protein 1;AT225;Nerve growth factor-induced protein A;NGFI-A;Transcription factor ETR103;Transcription factor Zif268;Zinc finger protein 225;Zinc finger protein Krox-24
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1F22, recombinant monoclonal
• descripción: recombinant, expressed in HEK 293 cells
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 57.51 kDa; observed mol wt ~80 kDa
• purificado por: using Protein A
• reactividad de especies: human
• reactividad de especies (predicha por homología): monkey
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: N-terminal half
• Nº de acceso Protein ID: NP_001955
• Nº de acceso UniProt: P18146
• categoría alternativa más sostenible: , Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... EGR1(1958)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 1F22 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Early growth response protein 1 (EGR1). It targets an epitope within 23 amino acids from the N-terminal half.

Inmunógeno

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal half of human Early growth response protein 1 (EGR1).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EGR1 in HeLa cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected EGR1 in PC3 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected EGR1 in human prostate tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected EGR1 in PC3 cells.

Affinity Binding Assay: A representative lot of this antibody bound EGR1 with a KD of 1.3 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Early growth response protein 1 (UniProt: P18146; also known as EGR-1, AT225, Nerve growth factor-induced protein A, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein Krox-24) is encoded by the EGR1 (also known as KROX24, ZNF225) gene (Gene ID: 1958) in human. EGR1 is a member of the EGR C2H2-type zinc-finger protein family that serves as a transcriptional regulator. It recognizes and binds to the DNA sequence 5′-GCG(T/G)GGGCG-3′(EGR-site) in the promoter region of target genes and regulates their transcription. It contains three C2H2-type Zinc fingers (a 338-362, 368-390, 396-418). The first, most N-terminal zinc finger binds to the 3′-GCG motif, the middle zinc finger interacts with the central TGG motif, and the C-terminal zinc finger binds to the 5′-GCG motif. It is shown to binds double-stranded target DNA, irrespective of the cytosine methylation status. It displays reduced affinity for target DNA where cytosines have been oxidized to 5-hydroxymethylcytosine and does not bind target DNA where cytosines have been oxidized to 5-formylcytosine or 5-carboxylcytosine. EGR1 plays an important role in regulating the response to growth factors, DNA damage, and ischemia. It is reported to activate the expression of p53/TP53 and TGFB1, and thereby prevents tumor formation and is shown to be essential for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. It is also regulates the expression of proteins such as IL-1b and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zandarashvili, L., et al. (2015). FEBS Lett. 589(15); 1748-1753; Hashimoto, H., et al. (2014). Genes Dev. 28(20); 2304-2313).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable