ZRB1630

Anti-EAAT2 Antibody, clone 1K8 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Excitatory amino acid transporter 2, Glutamate/aspartate transporter II, Sodium-dependent glutamate/aspartate transporter 2, Solute carrier family 1 member 2
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• clon: recombinant monoclonal
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 62.1 kDa; observed mol wt ~58 kDa
• reactividad de especies: mouse, rat, human
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso UniProt: P43004
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Especificidad

Clone 1K8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects excitatory amino acid transporter 2 (EAAT2). It targets an epitope within 20 amino acids from the C-terminal region.

Inmunógeno

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Excitatory amino acid transporter 2 (EAAT2).

Aplicación

Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EAAT2 in Rat Brain tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected EAAT2 in human and mouse Brain tissue lysates.Affinity Binding Assay: A representative lot of this antibody bound EAAT2 peptide with a KD of 5.8 x 10-7 in an affinity binding assay.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected EAAT2 in Rat astrocytes.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected EAAT2 in human brain and rat brain tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-EAAT2, clone 1K8 ZooMAb, Cat. No. ZRB1630, is a Rabbit monoclonal antibody that specifically targets EAAT2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Descripción de destino

Excitatory amino acid transporter 2 (UniProt: P43004; also known as Glutamate/aspartate transporter II, Sodium-dependent glutamate/aspartate transporter 2, Solute carrier family 1 member 2, EAAT-2) is encoded by the SLC1A2 (also known as EAAT2, GLT1) gene (Gene ID: 6506) in human. EAAT-2 is a multi-pass homotrimeric membrane protein serves as a major sodium-dependent, high-affinity transporter that moves glutamate back into the cell following neurotransmission. It is expressed predominantly in astroglial cells where it is responsible for about 90% of total glutamate uptake and maintains extracellular glutamate below excitotoxic levels. EAAT-2 functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion. EAAT-2 contains 6 cytoplasmic domains, 5 extracellular domains, and 8 transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 1.5 to 1.8 nm, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move. Mutation in SLC1A2 gene can cause early infantile epileptic encephalopathy, a heterogeneous group of severe childhood onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kim, K., et al. (2011). J. Cell Physiol. 226(10); 2484-2493).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable