ZRB1611

Anti-STX17 Antibody, clone 1B3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Syntaxin 17
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1B3, recombinant monoclonal
• descripción: recombinant, expressed in HEK 293 cells
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 33.4 kDa; observed mol wt ~38 kDa
• purificado por: using Protein A
• reactividad de especies: mouse, human
• reactividad de especies (predicha por homología): monkey, bovine
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: N-terminal cytoplasmic domain
• Nº de acceso Protein ID: NP_060389
• Nº de acceso UniProt: P56962
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 1B3 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Syntaxin 17 (STX17). It targets an epitope within 20 amino acids from the N-terminal, cytoplasmic domain.

Inmunógeno

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal, cytoplasmic domain of human Syntaxin17 (STX17).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected STX17 in A431 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected STX17 in lysates from HepG2 cells, mouse testis tissue, and C2C12 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected STX17 in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected STX17 in A431 and HeLa cells..

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Descripción de destino

Syntaxin-17 (UniProt: P56962; also known as STX17) is encoded by the STX17 gene (Gene ID: 55014) in human. STX17 is a multi-pass membrane protein with two cytoplasmic domains (aa 2-228 and 276-302), two helical domains (aa 229-249 and 255-275), and one lumenal domain (aa 250-254). STX17 is as an autophagosomal soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) that has a hairpin-type structure mediated by two transmembrane domains, each containing glycine zipper motifs. This structure contributes to its specific localization to completed autophagosomes. Mutations in the glycine residues on the glycine zipper-like motifs is shown to impair its localization to autophagosomes. Although STX17 is shown to localize to the endoplasmic reticulum and mitochondria under nutrient-rich conditions, it translocates to autophagosomes under condition of starvation. It is reported to interact with SNAP29 that is recruited to autophagosomes upon starvation, and VAMP8, the lysosomal SNARE. Depletion of either SNAP29 or VAMP8 is shown to block autophagic flux. STX17 also plays a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment and regulates transport between them. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Viret, C., and Faure, M. (2019). Trends Cell Biol. 29(11); 1-3; Itakura, E., et al. (2012). Cell. 151(6); 1256-1269).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable