ZRB1243

Anti-FGFR1 Antibody, clone 2J21 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 2J21, monoclonal; recombinant monoclonal
• Línea del producto: ZooMAb^® learn more
• Formulario: lyophilized
• mol peso: calculated mol wt ~91.87 kDa; observed mol wt ~80 kDa
• reactividad de especies: mouse, human
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso UniProt: P11362
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 2J21 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Fibroblast growth factor receptor 1 (FGFR1). It targets an epitope within 18 amino acids from the extracellular domain.

Inmunógeno

KLH-conjugated linear peptide corresponding to 18 amino acids from the extracellular domain of human Fibroblast growth factor receptor 1 (FGFR1).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FGFR1 in NIH3T3 cell lysate.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound FGFR1 peptide with a KD of 6.9 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FGFR1 in NIH3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-FGFR1, clone 2J21 ZooMAb^®, Cat. No. ZRB1243, is a recombinant Rabbit monoclonal antibody that specifically targets FGFR1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.

Descripción de destino

Fibroblast growth factor receptor 1 (UniProt: P11362; also known as EC:2.7.10.1, FGFR-1, Basic fibroblast growth factor receptor 1, BFGFR, bFGF-R-1, Fms-like tyrosine kinase 2, FLT-2, N-sam, Proto-oncogene c-Fgr, CD331, FGFR1) is encoded by the FGFR1 (also known as BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR) gene (Gene ID: 2260) in human. FGFR1 is a monomeric single-pass type I membrane protein that undergoes homodimerization upon ligand binding. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 22-376), a transmembrane domain (aa 377-397), and a cytoplasmic domain (aa 398-822). It serves as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. It is shown to be essential for normal mesoderm patterning and correct axial organization during embryonic development. FGFR1 is present in an inactive conformation in the absence of bound ligand and undergoes homodimerization and activation upon ligand binding and sequential autophosphorylation on tyrosine residues. After ligand binding, both receptor and ligand are rapidly internalized. Initial autophosphorylation at tyrosine 653 is shown to increase its tyrosine kinase activity by a factor of 50 to 100 and subsequently it is phosphorylated on tyrosine 583, 463, 766, 585, and 654, which causes a 10-fold increase in its kinase activity. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable