ZRB1099

Anti-ALDH1A1 Antibody, clone 4J6, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: rabbit (recombinant)
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 4J6, recombinant monoclonal
• Línea del producto: ZooMAb^® learn more
• Formulario: lyophilized
• mol peso: calculated mol wt 54.86 kDa; observed mol wt ~54 kDa
• reactividad de especies: human
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable (peptide)
• isotipo: IgG
• Nº de acceso UniProt: P00352
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ALDH1A1(216)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Especificidad

Clone 4J6 ZooMAb is a rabbit recombinant monoclonal antibody that specifically detects human Retinal Dehydrogenase 1. It targets an epitope within 19 amino acids from the C-terminal region.

Inmunógeno

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human Retinal dehydrogenase 1.

Aplicación

Anti-ALDH1A1, clone 4J6 ZooMAb , Cat. No. ZRB1099, is a highly specific rabbit recombinant monoclonal antibody that targets ALDH1A1 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected ALDH1A1 in lysates from HepG and human liver cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ALDH1A1 in human stomach and human liver tissue sections.

Immunoprecipitation Analysis: 3 μg from a representative lot immunoprecipitated ALDH1A1 in HepG2 cell lysate.

Immunofluorescence Analysis: A 1:10-1:100 dilution from a representative lot detected ALDH1A1 in human stomach and human liver tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Retinal dehydrogenase 1 (UniProt: P00352; also known as RALDH 1, RalDH1, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic) is encoded by the ALDH1A1 (also known as ALDC, ALDH1, PUMB1) gene (Gene ID: 216) in human. Retinal dehydrogenase 1 is a ubiquitously distributed cytosolic enzyme that serves as a detoxification enzyme and generates retinoic acid. It catalyzes the oxidation of retinal to retinoic acid that is essential for differentiation, development, and survival of dopaminergic neurons. It can also play a role in cellular defense against oxidative stress by oxidizing 4-hydroxynonenal (4-HNE) produced during lipid peroxidation. It is also involved in several other biological processes, such as stem cell maintenance, UV-radiation resistance, melanogenesis, and spermatogenesis, by catalyzing the conversion of specific aldehydes to their corresponding carboxylic acids. Retinal dehydrogenase 1 contains multiple NAD binding sites. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Paval, D., et al. (2017). Clin. Psychopharmacol. Neurosci. 5(3); 229-236).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable