ZMS1221

Anti-LAMP2 Antibody, clone H4B4 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: CD107 antigen-like family member B, CD107b, LAMP-2, LGP-96, Lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein 2
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: H4B4, recombinant monoclonal
• descripción: recombinant, expressed in HEK 293 cells
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 44.9 kDa; observed mol wt ~105 kDa
• purificado por: using protein G
• reactividad de especies: human
• envase: antibody small pack of 25
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG1κ
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: NP_001116078
• Nº de acceso UniProt: P13473
• categoría alternativa más sostenible: Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: human ... LAMP2(3920)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone H4B4 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects LAMP-2.

Inmunógeno

Human adherent peripheral blood cells.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected LAMP2 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected LAMP2 in lysate from U937 cells and 1:1,000 dilution in lysate from Jurkat cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected LAMP2 in Human liver tissue sections.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected LAMP2 in one million U937 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Lysosome-associated membrane glycoprotein 2 (UniProt: P13473; also known as LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, LGP-96, CD107b) is encoded by the LAMP2 gene (Gene ID: 3920) in human. LAMP-2 is a single-pass type I, heavily glycosylated, membrane protein found in plasma membrane, endosomes, and lysosomes. It is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form that contains a lumenal domain (aa 29-375), a transmembrane domain (aa 376-399), and a short cytoplasmic tail (aa 400-410). It is an important component of lysosomal membranes and is involved in both autophagy and lysosomal membrane permeabilization. LAMP-2 plays an important role in chaperone-mediated autophagy. It is required for the fusion of autophagosomes with lysosomes during autophagy. It also plays a role in lysosomal protein degradation in response to starvation. It functions by binding target proteins, such as GAPDH and MLLT11, and targets them for lysosomal degradation. In cancer cells, chronic acidosis-induced upregulation of LAMP-2 has been reported that protects these cells from acid-induced hydrolysis and promotes their survival via chaperone-mediated autophagy. Loss of LAMP-2 is reported to aggravate oxidative stress through the obstruction of reactive oxygen species clearance. Three different isoforms of LAMP-2 have been described that are produced by alternative splicing. Mutations in LAMP2 gene have been linked to Danon disease that is characterized by cardiomyopathy, vascular myopathy, and mental retardation. It is often associated with accumulation of glycogen in muscle and lysosomes. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Cui, L., et al. (2020). Front. Cell Dev. Biol. 8; 31; Qin, X., et al. (2017). Biochem. Biophys. Res. Commun. 488(1); 177-181; Cuervo, AM., and Dice, JF. (2000). J. Cell Sci. 113(24); 4441-4450).

Forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitución

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable