ZMS1188

Anti-Perforin-1 Antibody, clone dG9 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Cytolysin, Lymphocyte pore-forming protein, P1, PFP
• Código UNSPSC: 12352203
• NACRES: NA.43
• conjugado: unconjugated
• application: FACS; ICC; WB
• clon: dG9, recombinant monoclonal
• reactividad de especies: human
• técnicas: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: dG9, recombinant monoclonal
• descripción: recombinant, expressed in HEK 293 cells
• Línea del producto: ZooMAb^® learn more
• Formulario: lyophilized
• mol peso: calculated mol wt 61.38 kDa
• purificado por: using protein G
• reactividad de especies: human
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG2bκ
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: NP_001076585
• Nº de acceso UniProt: P14222
• categoría alternativa más sostenible: Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone dG9 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Perforin-1.

Inmunógeno

Purified granules from the human cytotoxic large granular lymphocytes lymphoma line YT.

Aplicación

Quality Control Testing

Evaluated by Western Blotting with His/SUMO-tagged recombinant Human Perforin-1.

Western Blotting Analysis: A 1:500 dilution of this antibody detected His/SUMO-tagged recombinant Human Perforin-1.

Tested Applications

Flow Cytometry Analysis: 1 µg from a representative lot detected Perforin-1 in one million Human peripheral blood mononuclear cells (PBMC).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Perforin-1 in SW480 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Perforin-1 (UniProt: P14222; also known as P1, Cytolysin, Lymphocyte pore-forming protein, PFP) is encoded by the PRF1 (also known as PFP) gene (Gene ID: 5551) in human. Perforin-1 is a multi-pass membrane glycoprotein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature form that contains two transmembrane domains (aa 129-149 and 257-279). It is stored in cytolytic granules of cytolytic T-lymphocytes and is secreted into the cleft between T-lymphocyte and target cell. Perforin has a thin KEY^®-shaped structure consisting of an N-terminal membrane attack complex perforin-like (MACPF)/ cholesterol dependent cytolysin (CDC) domain (aa 27-375), which is followed by an EGF domain (aa 376-408) that together with the eXTReme^™ C-terminal sequence forms a central shelf-like structure.  Its C-terminal C2 domain (aa 397-519) mediates initial, Ca2+-dependent membrane binding. Perforin-1 serves as a pore-forming protein that plays a KEY^® role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. It also plays an important role in killing other cells that are recognized as non-self by the immune system. It can insert into the membrane of target cells in its calcium-bound form and can oligomerize and form large pores. Its membrane insertion and pore formation require a major conformation change. It promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Mutations in PRF1 gene have been linked to familial Hemophagocytic lymphohistiocytosis (FHL2), a rare disorder that is characterized by immune dysregulation with hypercytokinemia, defective function of natural killer cell, and massive infiltration of several organs by activated lymphocytes and macrophages. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Praper, T., et al. (2011). J. Biol. Chem. 286(4); 2946-2955; Law, RHP., et al. (2010). Nature. 468(7322). 447-451; Ando, K., et al. (1997). J. Immunol. 158(11); 5283-5291).

Forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitución

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

Affinity is a trademark of Mine Safety Appliances Co.

KEY is a registered trademark of Hanna Instruments

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

eXTReme is a trademark of Excel Scientific, Inc.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable