ZMS1130

Anti-GABRB2 Antibody, clone BD17 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Gamma-aminobutyric acid receptor subunit beta-2;GABA(A) receptor subunit beta-2
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: BD17, recombinant monoclonal
• Línea del producto: ZooMAb^® learn more
• Formulario: lyophilized
• mol peso: calculated mol wt 54.45 kDa; observed mol wt ~52 kDa
• purificado por: using protein G
• reactividad de especies: human, mouse
• reactividad de especies (predicha por homología): bovine, feline, rat, monkey
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG1κ
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: NM_000813.2
• Nº de acceso UniProt: P0C2W5
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: bovine ... GABRB2(540657)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone BD17 is a ZooMAb^® Mouse recombinant monoclonal antibody that specifically detects GABA A receptor β2.

Inmunógeno

Purified GABA A receptor from bovine cerebral cortex.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in Mouse liver membrane preparation.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected GABRB2 in Mouse liver membrane preparation.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GABRB2 in Human brain and Mouse brain tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GABRB2 in Human cerebellum tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Gamma-aminobutyric acid receptor subunit beta-2 (UniProt: P0C2W5; also known as GABA(A) receptor subunit beta-2) is encoded by the GABRB2 gene (Gene ID: 540657) in Bovine. GABA is the major inhibitory neurotransmitter in the central nervous system that inhibits the generation of the action potential of the neuron. It plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel. It is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and are involved in synaptogenesis, neurogenesis, and the regulation of neuronal plasticity and learning. These receptors differ in their pharmacological, electrophysiological, and biochemical properties. GABRB2 is a multi-pass membrane protein that plays an important role in the formation of the pentameric structure of the GABA A receptor. It is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 24-239), three transmembrane domains, and a cytoplasmic domain (aa 330-450). The extracellular domains contribute to synaptic contact formation. GABRB2 is highly expressed in the brain with abundance in the gray matter of neurons and glial cells. It is associated with cognitive function related to time perception. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Barki, M., and Xue, H. (2022). Gene. 809;146021; Ymer, S., et al. (1989). EMBO J. 8(6); 1665-1670).

Forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitución

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable