ZMS1078

Anti-CFTR Antibody, clone MM13-4 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Cystic fibrosis transmembrane conductance regulator;ATP-binding cassette sub-family C member 7;Channel conductance-controlling ATPase;cAMP-dependent chloride channel
• Código UNSPSC: 12352203

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: MM13-4, recombinant monoclonal
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 168.14 kDa; observed mol wt ~170 kDa
• purificado por: using protein G
• reactividad de especies: human
• reactividad de especies (predicha por homología): monkey, porcine
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• técnicas: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG1κ
• secuencia del epítopo: N-terminal
• Nº de acceso Protein ID: NM_000492
• Nº de acceso UniProt: P13569
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CFTR(1080)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone MM13-4 is a ZooMAb^® Mouse recombinant monoclonal antibody that specifically detect CFTR. It targets an epitope within 12 amino acids from the N-terminal, first cytoplasmic domain.

Inmunógeno

A linear peptide corresponding to 12 amino acids from the N-terminal, extracellular domain of human Cystic fibrosis transmembrane conductance regulator (CFTR).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CFTR in HT-29 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CFTR in CFTR transfected human bronchial epithelial cell lysate.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected CFTR in one million CFTR transfected human bronchial epithelial cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CFTR in CFTR transfected bronchial epithelial cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CFTR in HT-29 cell lysate.

Descripción de destino

Cystic fibrosis transmembrane conductance regulator (UniProt: P13569; also known as CFTR, ATP-binding cassette sub-family C member 7, Channel conductance-controlling ATPase, cAMP-dependent chloride channel) is encoded by the CFTR (also known as ABCC7) gene (Gene ID: 1080) in human. CFTR is a multi-pass membrane protein with seven cytoplasmic domains, twelve transmembrane domains, and six extracellular domains. CFTR is an epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis. It mediates the transport of chloride ions across the cell membrane. This channel is also reported to be permeable to bicarbonate ions. Its channel activity is coupled to ATP hydrolysis. It is shown to play an important role in airway fluid homeostasis and contributes to the regulation of the pH and the ion content of the airway surface fluid layer and thereby has an important role in defense against pathogens. CFTR channel is internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane. Mutations in CFTR gene are reported to cause Cystic fibrosis that is characterized by chronic bronchopulmonary disease with recurrent respiratory infections and pancreatic insufficiency. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Shah, VS., et al. (2016). Science. 351(6272):503-507; Shcheynikov, N., et al. (2004). J. Biol. Chem. 279(21); 21857-21865).

Forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1