ZMS1038

Anti-Phosphatidylserine Antibody, clone 1H6 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: PS
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1H6, recombinant monoclonal
• descripción: recombinant, expressed in HEK 293 cells
• Línea del producto: ZooMAb^® learn more
• Formulario: lyophilized
• purificado por: using protein G
• reactividad de especies: human
• reactividad de especies (predicha por homología): all
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: flow cytometry: suitable; immunocytochemistry: suitable
• isotipo: IgG
• categoría alternativa más sostenible: Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 1H6 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Phosphatidylserine.

Inmunógeno

Liposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol.

Aplicación

Quality Control Testing

Evaluated by Immunocytochemistry in Jurkat cells treated with Staurosporine.

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Phosphatidylserine in Jurkat cells treated with staurosporine (2 µg/ml; 8 h).

Tested Applications

Flow Cytometry Analysis: 1 µg from a representative lot detected Phosphatidylserine in one million Jurkat cells treated with Staurosporine (2 µg/ml; 8 h).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Phosphatidylserine (PS) is a phospholipid (1,2-diacyl-sn-glycerol-(3)-L-phosphoserine) found in the membrane of all plants and animals. It is highly concentrated in the brain and may make up about 10% of the lipid content of neurons. Neuronal PS is reported to play a role in maintaining nerve cell integrity, enhancing neurotransmitter signal efficiency, and improving nerve cell signal transmission. It is required for healthy nerve cell membranes and myelin. It is usually found on the inner side of the plasma membrane, but during early-stage apoptosis, it translocates to the outer leaflet of plasma membrane. FITC-labeled Annexin V can bind to the exposed PS, providing the basis of most annexin V-based apoptosis assays. Anti-Phosphatidylserine can be is used to detect the translocation of the membrane PS in cells undergoing apoptosis. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Glade, MJ., and Smith, K. (2015). Nutrition. 31(6); 781-786; Lee, SH., et al. (2013). Cell Death Diffren. 20(1); 64-76).

Forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitución

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable