Y2021

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, tryptophan, and adenine

• Sinónimos: Yeast Synthetic Drop-out Media Supplements
• Número MDL: MFCD01095679
• Código UNSPSC: 12352200
• NACRES: NA.85

Propiedades

• Nivel de calidad: 200
• formulario: powder
• técnicas: transformation: suitable
• aplicaciones: microbiology
• temp. de almacenamiento: room temp

Descripción

Aplicación

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine has been used in yeast two-hybrid screening.

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Otras notas

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Nota de preparación

Dissolve 1.39 g of supplement per liter of medium.

Información de seguridad

• Pictogramas: GHS05
• Palabra de señalización: Danger
• Frases de peligro: H318
• Consejos de prudencia: P280 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Dam. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable