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XNAB2R

Sigma-Aldrich

Extract-N-Amp™ Blood PCR Kit

sufficient for 1000 extractions, sufficient for 1000 amplifications

Sinónimos:

Blood direct PCR kit

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About This Item

Código UNSPSC:
41106303
NACRES:
NA.55

uso

sufficient for 1000 amplifications
 sufficient for 1000 extractions
 sufficient for 1000 reactions

Nivel de calidad

200

Características

dNTPs included
hotstart

técnicas

PCR: suitable

color

colorless

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

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Descripción general

The Extract-N-Amp™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.

Aplicación

Extract-N-Amp™ Blood PCR Kit has been used to extract DNA from genomes and dried blood spot samples. It has also been used for polymerase chain reaction (PCR).

Características y beneficios

  • Efficient 8 minute prep allows greater speed and throughput
  • No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
  • Simple, 3 step procedure with no special equipment required
  • Hot start antibody included for highly specific PCR amplification of genomic DNA
  • Compatible with multiple format (single tube or 96-well plate)
  • Can be used with whole blood or blood cards
  • Extract stable at 4 °C for at least 6 months

Principio

Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.

Otras notas

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Información legal

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • L3289Lysis Solution for BloodSDS
  • N9784Neutralization Solution for BloodSDS
  • P8115Extract-N-Amp™ PCR ReadyMix™ for BloodSDS

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N9784

Neutralization Solution for Blood, sufficient for 100 reactions, sufficient for 1000 reactions, for molecular biology

Código de clase de almacenamiento

10 - Combustible liquids

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Certificados de análisis (COA)

Lot/Batch Number
091K9296
091K9295

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Sandrine Romand et al.
Biotechnology and bioengineering, 113(5), 1094-1101 (2015-11-03)
Chinese Hamster Ovary (CHO) cells are widely used for the large-scale production of recombinant biopharmaceuticals. However, attempts to express IGF-1 (a mutated human Insulin-like growth factor 1 Ea peptide (hIGF-1Ea mut)) in CHO cells resulted in poor cell growth and
Mads Vilhelm Hollegaard et al.
Electrophoresis, 30(14), 2532-2535 (2009-07-30)
Stored surplus of dried blood spot (DBS) samples from neonatal screening programs constitute a vast potential for large genetic epidemiological studies. However, age of the samples and the small amounts of DNA available may limit their usage. In this study
Jun Shi et al.
Cancer research, 67(13), 6417-6424 (2007-07-10)
Idiopathic myelofibrosis (IM) is likely the consequence of both the acquisition of genetic mutations and epigenetic changes that silence critical genes that control cell proliferation, differentiation, and apoptosis. We have explored the effects of the sequential treatment with the DNA
Mads V Hollegaard et al.
BMC genomics, 10, 297-297 (2009-07-07)
Identification of disease susceptible genes requires access to DNA from numerous well-characterised subjects. Archived residual dried blood spot samples from national newborn screening programs may provide DNA from entire populations and medical registries the corresponding clinical information. The amount of
Nicholas Wong et al.
BioTechniques, 45(4), 423-424 (2008-10-16)
Sodium bisulfite treatment followed by PCR and DNA sequencing is widely considered the gold standard for the analysis of DNA methylation patterns. However, this technique generally requires substantial quantities of genomic DNA as starting material and is often associated with
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Artículos

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards

Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. This methodology can be used without modification to perform PCR on blood samples stored on FTA blood cards (Whatman) which removes the need for the laborious and costly washing procedures that the standard FTA protocol stipulates. This technique removes the variability between sample preparations and maximizes the number of PCR reactions that can be performed on individual FTA blood card samples.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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