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T6778

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Goat Anti-Rabbit IgG (whole molecule)–Tetramethylrhodamine isothiocyanate

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About This Item

Número MDL:
MFCD00162790
Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

goat

Nivel de calidad

200

conjugado

TRITC conjugate

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

secondary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

contiene

≥15 mM sodium azide as preservative

condiciones de almacenamiento

protect from light

técnicas

direct immunofluorescence: 1:400
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Descripción general

Binds all rabbit Igs.
IgG consists of four isotypes IgG1, IgG2, IgG3 and IgG4. It contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which is linked together by inter-chain disulfide bonds.

Aplicación

Anti-Rabbit IgG (whole molecule)−TRITC antibody produced in goat has been used in immunocytochemistry.
Conjugation of TRITC to Rabbit IgG (whole molecule) antibody enables the use in immunofluorescent techniques.

Acciones bioquímicas o fisiológicas

IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be associated with allergy. IgG mediates transplacental transport and participates in the secondary immune response. It has the longest serum half life.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Otras notas

Antibody adsorbed with human IgG.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4.

Nota de preparación

Adsorbed to reduce background staining with human samples.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
069M4896V
21190170
036M4785V
115M4840V
024M4802V

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Goeh Jung et al.
The Journal of cell biology, 186(4), 555-570 (2009-08-19)
The contractile vacuole (CV) complex in Dictyostelium is a tubulovesicular osmoregulatory organelle that exhibits extensive motility along the actin-rich cortex, providing a useful model for investigating myosin-dependent membrane transport. Here, we show that the type V myosin myoJ localizes to
Kamin J Johnson et al.
Biology of reproduction, 66(4), 992-1000 (2002-03-22)
In the seminiferous epithelium, morphologically diverse junctions mediate inter-Sertoli and Sertoli-germ cell adhesive contact and likely transmit signals between contacting cells. Defining the molecular composition of testicular cell-cell junctions is an important step in determining their function. Proteins belonging to
5-HT1B and 5-HT1D receptors in the human trigeminal ganglion: co-localization with calcitonin gene-related peptide, substance P and nitric oxide synthase
Hou M, et al.
Brain Research, 909(1-2), 112-120 (2001)
Daniel H Fine et al.
Infection and immunity, 73(4), 1947-1953 (2005-03-24)
Cells of the gram-negative periodontopathogen Actinobacillus actinomycetemcomitans express a surface-exposed, outer membrane autotransporter protein, designated Aae, which has been implicated in epithelial cell binding. We constructed a mutant strain of A. actinomycetemcomitans that contained a transposon insertion in the Aae
Martin Hasselblatt et al.
Cancer research, 69(6), 2219-2223 (2009-03-12)
The pathogenesis of choroid plexus papillomas, intraventricular papillary neoplasms most often occurring sporadically in children and young adults, remains poorly understood. To identify pathways operative in the development of choroid plexus papillomas, gene expression profiles obtained from laser-microdissected human choroid
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