T1819

Trizma^® hydrochloride solution

pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

• Sinónimos: Tris hydrochloride solution
• Código UNSPSC: 12161700
• NACRES: NA.25

Propiedades

• grado: BioPerformance Certified; for molecular biology
• Nivel de calidad: 200
• Formulario: liquid
• concentración: 1 M
• técnicas: cell culture | mammalian: suitable
• impurezas: DNase, RNase, Protease, none detected; bioburden, tested; endotoxin, tested; ≤5 ppm Heavy metals (as Pb)
• pH: 7.0
• intervalo de pH útil: 7.0-9.0
• Absorción: ≤0.05 at 290 at 40%

Descripción

Descripción general

Tris(hydroxymethyl)aminomethane (Tris) is a standard buffer in biological sciences and has a pH buffering range between 7.0 - 9.0. It is applicable in electrophoretic studies and capillary electrochromatography.

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Aplicación

Trizma^® hydrochloride solution has been used:

• as a buffer in reverse transcription and DNase I treatment reactions for RNA isolated from Toxoplasma gondii
• in the preparation of tris- ethylenediaminetetraacetic acid (EDTA) (TE) buffer for bacterial pellet resuspension for plasmid isolation studies
• for polylactic acid nanoparticle resuspension, storage, and pH-based stability studies

Información legal

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type ABEK (EN14387) respirator filter