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T1165

Sigma-Aldrich

Tris-Tricine-SDS Buffer 10× Concentrate

Sinónimos:

10X TTS Buffer, 10X Tris Tricine SDS running buffer, Tris Tricine SDS transfer buffer

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About This Item

Código UNSPSC:
41105319
NACRES:
NA.25

esterilidad

0.2 μm filtered

Nivel de calidad

formulario

liquid

pH

8.05-8.30 (25 °C in water, high purity, 1:10)

aplicaciones

diagnostic assay manufacturing

temp. de almacenamiento

2-8°C

Descripción general

Tris-Tricine-SDS (TTS) running buffer is the cathode (upper reservoir) buffer for SDS−polyacrylamide gel electrophoresis of proteins using the Schagger and von Jagow method. The Schagger and von Jagow method is designed for the separation of small molecular weight proteins. It differs from the Laemmli method in that the glycine is replaced with tricine and the gel contains 1M Tris-HCl, pH 8.45 instead of 0.375 M Tris-HCl, pH 8.9. Dilution of the 10X TTS buffer produces a 1X cathode buffer containing 100 mM Tris, 100 mM tricine and 0.1% SDS. Recommended running conditions is 125 volts.

Aplicación

Tris-Tricine-SDS Buffer 10× Concentrate is suitable for use as a running buffer for polyacrylamide based separation of bronchoalveolar lavage fluid (BALF).

Otras notas

1M Tris, 1M Tricine, 1% (w/v) SDS, pH approx. 8.2.

Nota de análisis

Tested for use as a cathode buffer for Tris-Tricine gel electrophoresis to separate low molecular weight proteins.

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Nonviral gene delivery to the lung with copolymer-protected and transferrin-modified polyethylenimine
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Biochim. Biophys. Acta Gen. Subj., 1573(1), 75-83 (2002)
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Alzheimer's disease (AD) is characterized by slow, progressive neurodegeneration leading to severe neurological impairment, but current drug development efforts are limited by the lack of robust, human-based disease models. Amyloid-β (Aβ) is known to play an integral role in AD
H Schägger et al.
Analytical biochemistry, 166(2), 368-379 (1987-11-01)
A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations
Rachel E Gerver et al.
Analytical chemistry, 86(21), 10625-10632 (2014-10-01)
We describe a microfluidic Western blot assay (μWestern) using a Tris tricine discontinuous buffer system suitable for analyses of a wide molecular mass range (6.5-116 kDa). The Tris tricine μWestern is completed in an enclosed, straight glass microfluidic channel housing
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
U K Laemmli
Nature, 227(5259), 680-685 (1970-08-15)

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