SRP5270

SIRT1, active, His tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Sinónimos: SIR2L1
• Número de CAS: 1391438-73-5
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in baculovirus infected Sf9 cells
• Ensayo: ≥70% (SDS-PAGE)
• Formulario: buffered aqueous glycerol solution
• actividad específica: 1573-2415 RLU/min·ng
• mol peso: ~84 kDa
• Nº de acceso NCBI: NM_012238
• aplicaciones: genomic analysis
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... SIRT1(23411)

Descripción

Descripción general

Sirtuin 1 (SIRT1) is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT1 being a member of class I. SIRT1 is a stress-response and chromatin-silencing factor. SIRT1 is also called as NAD-dependent deacetylase sirtuin-1. This gene is located on human chromosome 10q21.3.

Acciones bioquímicas o fisiológicas

Sirtuin 1 (SIRT1) plays an important role in neuronal proliferation and vascular cells. It also plays an athero-protective role. Mutations in the SIRT1 gene affects the metabolism, cholesterol and fat deposition. SIRT1 has the ability to intermediate the insulin resistance through an inflammatory process, reactive oxygen species, gluconeogenesis and adiponectin level. It is an NAD(+)-dependent histone deacetylase involved in various nuclear events such as transcription, DNA replication, and DNA repair. SIRT1 protein binds and deacetylates the p53 protein. Expression of wild type SIRT1 in human cells reduces the transcriptional activity of p53 indicating that SIRT1 is involved in the regulation of p53 function via deacetylation.

Forma física

Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol.

Nota de preparación

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Información de seguridad

• Pictogramas: GHS08,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H315,H319,H360D
• Consejos de prudencia: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Clasificaciones de peligro: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Código de clase de almacenamiento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable