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SRP5003

Sigma-Aldrich

AMPK (α2/β2/γ1), active, His tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sinónimos:

AMPK, AMPK2, AMPKG, MGC61468, MGC8666, PRKAA, PRKAA2, PRKAB2, PRKAG1

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

recombinante

expressed in baculovirus infected Sf9 cells

Línea del producto

PRECISIO® Kinase

Ensayo

≥70% (SDS-PAGE)

Formulario

buffered aqueous glycerol solution

actividad específica

238-322 nmol/min·mg

mol peso

~36 kDa (B2)
 ~40 kDa (G1)
 ~69 kDa (A2)

Nº de acceso NCBI

NM_002733
NM_005399
NM_006252

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Información sobre el gen

human ... PRKAA2(5563) , PRKAB2(5565) , PRKAG1(5571)

Descripción general

AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.

Forma física

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Nota de preparación

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Información legal

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de señalización

Danger

Frases de peligro

H315,H319,H360D

Clasificaciones de peligro

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
R100-2

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Qin Zhao et al.
Molecular cell, 77(1), 95-107 (2019-10-20)
GTP cyclohydrolase I (GTPCH), 6-pyruvoyltetrahydropterin synthase (PTPS), and sepiapterin reductase (SR) are sequentially responsible for de novo synthesis of tetrahydrobiopterin (BH4), a known co-factor for nitric oxide synthase (NOS). The implication of BH4-biosynthesis process in tumorigenesis remains to be investigated.
Barbara B Kahn et al.
Cell metabolism, 1(1), 15-25 (2005-08-02)
The AMP-activated protein kinase (AMPK) is an evolutionarily conserved sensor of cellular energy status, and recent data demonstrate that it also plays a critical role in systemic energy balance. AMPK integrates nutritional and hormonal signals in peripheral tissues and the
D Grahame Hardie
Journal of cell science, 117(Pt 23), 5479-5487 (2004-10-29)
The AMP-activated protein kinase (AMPK) cascade is a sensor of cellular energy status. Whenever the cellular ATP:ADP ratio falls, owing to a stress that inhibits ATP production or increases ATP consumption, this is amplified by adenylate kinase into a much
Ting Wang et al.
Nature cell biology, 19(7), 833-843 (2017-06-20)
Chromatin-associated fumarase (FH) affects histone methylation via its metabolic activity. However, whether this effect is involved in gene transcription remains to be clarified. In this study, we show that under glucose deprivation conditions, AMPK phosphorylates FH at Ser75, which in
Xiang-Rong Li et al.
Cell death discovery, 7(1), 11-11 (2021-01-16)
Besides their original regulating roles in the brain, spinal cord, retina, and peripheral nervous system for mediating fast excitatory synaptic transmission, glutamate receptors consisting of metabotropic glutamate receptors (GluRs) and ionotropic glutamate receptors (iGluRs) have emerged to have a critical

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