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SRP5001

Sigma-Aldrich

AKT1, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sinónimos:

MGC99656, PKB, PRKBA, RAC, RAC-α

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

recombinante

expressed in baculovirus infected Sf9 cells

Línea del producto

PRECISIO® Kinase

Análisis

≥70% (SDS-PAGE)

formulario

buffered aqueous glycerol solution

actividad específica

103-139 nmol/min·mg

mol peso

~85 kDa

Nº de acceso NCBI

NM_005163

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Información sobre el gen

human ... AKT1(207)

Descripción general

v-akt murine thymoma viral oncogene homolog 1 (AKT1) is a Ser/Thr protein kinase, and is also called protein kinase B (PKB). This protein is activated when it is phosphorylated at serine 473 and threonine 308. This gene is localized on human chromosome 14q32.32.

Acciones bioquímicas o fisiológicas

v-akt murine thymoma viral oncogene homolog 1 (AKT1) is responsible for the phosphorylation and cytoplasmic localization of CKDN1A (cyclin-dependent kinase (CDK) inhibitor 1, also referred to as p21). However, p21 is one of the CDKNs which normally resides in the nucleus and its cytoplasmic localization, mediated by AKT1, is common to various cancers where it suppresses proteins essential for apoptosis. Studies show that up-regulation of AKT1 serves as a poor prognostic marker in breast cancer, which however, depends upon factors including population, hormone receptor status, and hormonal or trastuzumab therapy status.

Forma física

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Nota de preparación

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Información legal

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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The prognostic value of phosphorylated Akt in breast cancer: a systematic review.
Yang ZY
Scientific Reports (2015)
Gene expression profiling of mantle cell lymphoma cells reveals aberrant expression of genes from the PI3K-AKT, WNT and TGFbeta signalling pathways.
Rizzatti EG
British Journal of Haematology (2005)
Wei Luo et al.
Nature immunology, 20(6), 736-746 (2019-04-24)
B cell antigen receptor (BCR) and CD40 signaling are rewired in germinal center (GC) B cells (GCBCs) to optimize selection for high-affinity B cells. In GCBC, BCR signals are constrained, but the mechanisms are not well understood. Here we describe
P J Coffer et al.
The Biochemical journal, 335 ( Pt 1), 1-13 (1998-09-22)
While a plethora of extracellular molecules exist that modulate cellular functions via binding to membrane receptors inside the cell, their actions are mediated by relatively few signalling mechanisms. One of these is activation of phosphatidylinositol 3-kinase (PI-3K), which results in
K E Anderson et al.
Current biology : CB, 8(12), 684-691 (1998-06-25)
Protein kinase B (PKB) is involved in the regulation of apoptosis, protein synthesis and glycogen metabolism in mammalian cells. Phosphoinositide-dependent protein kinase (PDK-1) activates PKB in a manner dependent on phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), which is also needed for the translocation

Artículos

Oncogenes and Tumor Suppressors Reprogram Metabolism

We present an article about how proliferating cells require the biosynthesis of structural components for biomass production and for genomic replication.

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